An optimized CT-dense agent perfusion and micro-CT imaging protocol for chick embryo developmental stages.

Compared to classical techniques of morphological analysis, micro-CT (µ-CT) has become an effective approach allowing rapid screening of morphological changes. In the present work, we aimed to provide an optimized micro-CT dense agent perfusion protocol and µ-CT guidelines for different stages of chick embryo cardiogenesis. Our study was conducted over a period of 10 embryonic days (Hamburger-Hamilton HH36) in chick embryo hearts. During the perfusion of the micro-CT dense agent at different developmental stages (HH19, HH24, HH27, HH29, HH31, HH34, HH35, and HH36), we demonstrated that durations and volumes of the injected contrast agent gradually increased with the heart developmental stages contrary to the flow rate that was unchanged during the whole experiment. Analysis of the CT imaging confirmed the efficiency of the optimized parameters of the heart perfusion.

In vitro and in vivo study on fine-grained Mg-Zn-RE-Zr alloy as a biodegradeable orthopedic implant produced by friction stir processing.

Magnesium alloys containing biocompatible components show tremendous promise for applications as temporary biomedical devices. However, to ensure their safe use as biodegradeable implants, it is essential to control their corrosion rates. In concentrated Mg alloys, a microgalvanic coupling between the α-Mg matrix and secondary precipitates exists which results in increased corrosion rate. To address this challenge, we engineered the microstructure of a biodegradable Mg-Zn-RE-Zr alloy by friction stir processing (FSP), improving its corrosion resistance and mechanical properties simultaneously. The FS processed alloy with refined grains and broken and uniformly distributed secondary precipitates showed a relatively uniform corrosion morphology accompanied with the formation of a stable passive layer on the alloy surface. In vivo corrosion evaluation of the processed alloy in a small animal model showed that the material was well-tolerated with no signs of inflammation or harmful by-products. Remarkably, the processed alloy supported bone until it healed till eight weeks with a low in vivo corrosion rate of 0.7 mm/year. Moreover, we analyzed blood and histology of the critical organs such as liver and kidney, which showed normal functionality and consistent ion and enzyme levels, throughout the 12-week study period. These results demonstrate that the processed Mg-Zn-RE-Zr alloy offers promising potential for osseointegration in bone tissue healing while also exhibiting controlled biodegradability due to its engineered microstructure. The results from the present study will have profound benefit for bone fracture management, particularly in pediatric and elderly patients.

An operational guide to resin 3D printing of geological macromodels.

Stereolithography (SLA) is a form of 3D printing that is based on the curing of resin under UV light. There are a wide variety of 3D resin printers on the market that all follow the same general procedure. First, a slicing program is used to slice the model in a sequence of thin layers. The model will be printed in this sequence of layers after it is exported in a format recognizable by a 3D printer. In addition to this main function, slicing programs offer additional features to manipulate the model, adjust print settings, and add model supports. Next, after the printer is set up, the sliced model is loaded onto the printer and fabricated. Once the print is complete, the model can be washed, cured and sanded/polished to the desired finish. In this work, we utilize SLA 3D printing to print geological macromodels, to be utilized in flooding experiments. Images captured from the flooding experiments were then incorporated in a set of visual learning exercises for undergraduate students to enhance the study of immiscible fluid flow in porous media. SLA printing was selected in this use case as it provides important advantages over other common 3D printing technologies (e.g. Fused Depositional Modelling: FDM), such as high print resolvability of sub-millimeter scale pore geometry and a high degree of transparency within the resultant printed models. Overall, this method was found to:•Provide an engaging learning experience for undergraduate students, as the captured flooding experiment image time series allowed students to directly visualize often obtuse fluid flow processes in porous media.•Be easily reproducible: after completing an initial print the method can be reproduced for many different pore networks, allowing for a wide array of comparative studies and learning exercises to be developed.

Blood Flow Disturbance and Morphological Alterations Following the Right Atrial Ligation in the Chick Embryo.

Collectively known as congenital heart defects (CHDs), cardiac abnormalities at birth are the most common forms of neonatal defects. Being principally responsible for the heart's pumping power, ventricles are particularly affected by developmental abnormalities, such as flow disturbances or genomic defects. Hypoplastic Right Heart Syndrome (HRHS) is a rare disease where the right ventricle is underdeveloped. In this study, we introduce a surgical procedure performed on chick embryo, termed right atrial ligation (RAL) for disturbing hemodynamics within the right heart aiming in order to generate an animal model of HRHS. RAL is a new surgical manipulation, similar to the well-studied left atrial ligation (LAL) surgery but it induces the hemodynamic change into the right side of the heart. After inducing RAL, We utilized techniques such as Doppler ultrasound, x-ray micro-CT, histology, and computational fluid dynamics (CFD) analysis, for a comprehensive functional and structural analysis of a developing heart. Our results displayed that RAL does not induce severe flow disturbance and ventricular abnormalities consistent with clinical findings. This study allows us to better understand the hemodynamics-driven CHD development and sensitivities of ventricles under disturbed flows.

Inertia Controlled Capillary Pressure at the Juncture between Converging and Uniform Channels.

In this research, we reveal the transient behavior of capillary pressure as the fluid-fluid interface travels across the juncture between a converging and uniform capillary, via high-resolution CFD (Computational Fluid Dynamics) simulations. Simulations were performed at different wetting conditions (strong-wet and intermediate-wet) and capillary wall convergence angles. Our results demonstrate that as the angle of convergence increases, capillary pressure at the junction decreases commensurately. Moreover, in contrast to strong-wet conditions, the profile of capillary pressure at the converging-uniform capillary juncture under intermediate-wet conditions is highly non-monotonic, being characterized by a parabola-like form. This non-monotonic behavior is a manifestation of strong inertial forces governing dynamic fluid-fluid interface morphology. This yields conditions that promote the advancement of the fluid-fluid interface, as inertial forces partially nullify the capillary pressure required for the immiscible interface to enter the uniform capillary. In addition to numerical analysis detailed above, a novel theoretical stability criteria that is capable of distinguishing between stable (capillary dominated) and unstable (inertia dominated) interfacial regimes at the converging-uniform capillary juncture is also proposed. In summary, this fundamental study offers new insights into the interface invasion protocol, and paves the way for the re-evaluation of capillary junction controlled interfacial dynamics.